Zinc (Zn) Analysis in Milk by Microwave Oven Digestion and Differential Pulse Anodic Stripping Voltametry (DPASV) Technique
Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences (AIIMS), New Delhi, India - 110029, +91-9891564641
Milk is very important component of human diet. The presence of over limit of heavy metal in milk may create significant health problems. In the present study, the direct determination of Zinc (Zn) heavy metal in milk samples of different brands was carried out by differential pulse anodic stripping Voltammetric technique at Hanging Mercury Drop Electrode (HMDE). Milk samples were processed by microwave oven digestion using HP/VHP Vessels and TFM Liners and nitric acid (HNO3).Determination of Zn was made in acetate buffer (pH 4.6) with a sweep rate (scan rate) of 59.5 mV/s and pulse amplitude 50mV by HMDE by standard addition method. The solution was stirred during pre-electrolysis at -1150mV (vs. Ag/AgCl) for 90 seconds and the potential was scanned from -1150V to +100V (vs. Ag/AgCl). The zinc ions were deposited by reduction at -1150 mV on HMDE. The stripping current arising from the oxidation of metal was correlated with the concentration the metal in the sample. .As a result the minimum level of Zn observed in the milk sample of different brands was determined as 2.28 mgL−1.
Key words: Anodic stripping Voltametry / Hanging mercury dropping electrode / Heavy metals / Zinc / milk
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