Rate of change in concentration of organic compounds and nutrients in municipal and dairy wastewater treated in full technical SBR scale

Municipal WWTP often receive the industrial wastewater, including dairy sewage. The problem of sewage treatment from this sector of economy is not completely solved, especially in plants of medium and small milk processing capacity. The aim of the study was to determine the rate of change in organic compounds and nutrients in municipal wastewater with the participation of dairy products during the processing phases of SBR-type reactor in a technical scale. The study was conducted in WWTP in Sokolka. Rate of changes in the concentrations of organic compounds, nitrogen and phosphorus in wastewater during the processing phases of SBR reactor was determined. Presence of dairy sewage (13% volume participation) affected the decrease in the rate of changes in organic compounds concentrations defined as BOD5 during all processing phases of SBR reactor. Significant influence of dairy sewage on rates of change in nitrogen and phosphorus compounds concentrations during filling, aeration, stirring, sedimentation and decantation phases, was found.


Introduction
In municipal wastewater treatment technology, the activated sludge in a batch SBR system is often used.Wastewater treatment is carried out in a single-chamber bioreactor, in which during the alternating phases, processes of integrated removal of carbon, nitrogen and phosphorus occur, as well as the distribution of treated wastewater from the process medium, takes place.There are few reports [1][2][3] in the literature on the rate of change in organic compounds and nutrients during SBR processing phases.
Municipal sewage treatment plants often receive the industrial wastewater, including dairy sewage.The problem of sewage treatment from this sector of economy is not completely solved, especially in plants of medium and small milk processing capacity.Dairy wastewater is characterized by high load of COD and BOD 5 .This includes dissolved and crystallized fats (glycerol, triglycerides), sugars (lactose), and protein (casein) in colloidal form and as a clot.Shares of these substances can vary.Quantity of dairy sewage is characterized by high seasonal variations (20% increase in spring and summer).Additional oscillations relate to the daily and hourly shift work dependent on the size, production profile, and shifts at milk processing plant [4][5][6][7][8][9][10][11][12].Biological treatment of dairy wastewater is high-efficient due to the significant share of easily biodegradable substances [13][14][15][16].It is possible to co-treat the dairy and municipal wastewater to achieve the desired C:N:P ratio in order to carry out highly effective denitrification and biological phosphorus removal.However, analysis of the up-to-date knowledge showed that there is a lack of data on the effects of dairy sewage on the efficiency of municipal wastewater treatment in sequential chambers.
The aim of the study was to determine the rate of change in organic compounds and nutrients in municipal wastewater with the participation of dairy products during the processing phases of SBR-type reactor in a technical scale.

Area of study
The study was conducted in mechanical-biological treatment plant in Sokolka.The facility consists of mechanical sewage treatment station (blocked device of type Rotomat Huber Ro5/780/2 with capacity of 120 l•s -1 equipped with the cylindrical sieve with a diameter of 780 mm and 2 mm clearance holes, sand traps purged from the scrubber sand RoSF4tC, fat separator, and press for dewatering of remains).Mechanically treated wastewater flows into the intermediate pumping station.They are pumped into the averaging two-chamber retention tank with an active volume of 1800 m 3 .This tank is equipped with 2 hyperboidal stirrers of type HCMA with aeration grate and in mixer 2 hyperboidal stirrers of type HCM.There is the possibility of dosing the coagulants.Wastewater is pumped from the tank to the sequential biological reactors (SBR).The process of biological treatment is carried out in the cyclic system, in four cylindrical chambers of the active capacity of 1500 m 3 , equipped with hyperboidal stirrers of type HCMA.Duration of a single cycle is 8 hours 10 minutes (filling phase -0.7 h, aeration phase -4 h, the stirring phase -1 h, sedimentation phase -1.5 h, decantation phase -1 h).Oxygen conditions vary over a range from 1.8 to 4 g O 2 •m -3 .The maximum daily capacity of the plant is 6000 m 3 , and the equivalent population (PE) 25070.The object receives domestic and industrial wastewater from the milk processing plant.Dairy Cooperative in Sokolka discharges sewage to municipal sewage treatment plant after the pretreatment process.Table 1 presents types of wastewater and sludge subjected to physicochemical determination.Table 3 presents selected data on the production profile of the dairy processing plant.

Analytical Methods
The scope of analytical determinations made in samples included analysis of the following indicators: BOD 5 , COD, TOC, N tot , P tot -in wastewater; V, MLSS, SVI -in activated sludge.Analyses were carried out in accordance with APHA (2012) [17].Based on the study results, calculations of carbon, nitrogen and phosphorus removal efficiency (total and partial), as well as the rate of change in parameters during the processing phases of SBR reactor, were carried out.Total removal efficiency was calculated from the difference of a given parameter concentration at the inlet (C O ) and outlet (C).Efficiency of the partial of organic carbon, nitrogen and phosphorus removal from wastewater was calculated from the difference of a given parameter concentration at the start (C X ) and end (C Y ) of the treatment phase.
Rate of changes in the concentrations of organic compounds, nitrogen and phosphorus in wastewater (r V ) during the processing phases of SBR reactor was determined from the formula: r V -rate of the parameter change in wastewater, mg•dm A -concentration at the start of processing phase, mg•dm -3 B -concentration at the end of processing phase, mg•dm -3 T -durability of processing phase, h.
To determine the rate of change in parameters in wastewater relative to the concentration of activated sludge in SBR reactor (r X ) for particular processing phases, following dependence was applied: •h -1 X p -concentration of activated sludge in SBR at the start of processing phase, g DM •dm -3 X k -concentration of activated sludge in SBR at the end of processing phase, g DM •dm -3 .To calculate the rates (r V , r X ), a simplification was assumed that concentrations of organic compounds and nutrients at the end of the processing phase are equal to the concentrations at the start of the subsequent SBR processing phase.It was also assumed that changes in biomass of the activated sludge during an 8-hour SBR cycle are not significant [1,2,14].Accordingly, the equation takes the following form: X -concentration of activated sludge in SBR, g DM •dm -3 .In order to determine the influence of dairy sewage on the rate of change in the concentrations of organic compounds and nutrients, municipal wastewater (collected before connecting with a stream of dairy sewage) was purified in a model SBR reactor presented in paper by Struk-Sokołowska and Ignatowicz [18], according to the methodology described in the publications by Struk-Sokołowska [2,18].Municipal wastewater treatment in a model SBR reactor was performed in this study.Processing parameters and the activated sludge were the same as in the wastewater treatment plant in Sokolka.

Results
Table 4 presents characteristics of activated sludge in Sokolka.134.2

Rates of parameters change in wastewater in various stages of treatment
The rates of changes (r V and r X ) in particular parameters of municipal wastewater with 13% share of dairy sewage are presented in Table 5.
In mixed filling phase in SBR in Sokolka wastewater is mixed with the biomass.It initiates biological reactions, degrades the organics by using residual oxygen.Denitrification may occur in this fill.Average rate of total nitrogen removal during 40-minute filling phase in SBR content was 64.3 mg•dm -3 •h -1 (in reference to the activated sludge biomass concentration was 16.92 mg•g DM •h -1 ).During this phase, some decrease in the total phosphorus concentration was recorded.Rate of phosphorus removal was 6.4 mg ).Average rate of organic matter removal expresses as BOD 5 during filling phase amounted to 668.6 mg•dm -3 •h -1 (in relation to the biomass -176 mg•g DM •h -1 ).Average rate (r V ) of organic matter removal determined by parameter COD during dephosphatation in SBR was 922.
Aeration phase in SBR in Sokolka may be mixed react or aerated react type.Mixed react allows anoxic condition to achieve de-nitrification and aerated react allows aerobic condition during aerated fill to achieve complete nitrification.The aeration phase lasting 240 minutes cause the removal of the total nitrogen from wastewater at mean rate of 1.5 mg•dm -3 •h -1 (0.39 mg•g DM •h -1 ).It should be underlined that this rate during initial 60 minutes amounted to 7.8 mg•dm -3 •h -1 (2.05 mg•g DM •h -1 ), while during the subsequent 180 minutes, it decreased up to 0.5 mg•dm -3 •h -1 (0.13 mg•g DM •h -1 ) or the increase of the total nitrogen concentration at the rate of 1.2 mg•dm -3 •h -1 (0.32 mg•g DM •h -1 ), was recorded.4

EKO-DOK 2017
With the beginning of the aeration phase, the concentration of nitrogen increased as a result of nitrification, before the settling phase.Before the decantation phase the concentration of nitrogen decreased, which may result from denitrification effected by denitrificators which contain organic matter in their cells [3].This phase caused the total phosphorus removal from wastewater at the average rate of 1.1 mg•dm -3 •h -1 (over 2 mg•dm -3 •h -1 during initial 120 minutes).It should be emphasized that between 120 and 180 minute of the aeration phase, sudden release of total phosphorus at the rate of 3.2 mg•dm -3 •h -1 was observed, and then between 180 and 240 minute of that phase, total phosphorus concentration decrease at the rate of 3 mg•dm -3 •h -1 .In order to remove phosphorus biologically, alternate aerobic and anaerobic conditions have to be ensured.During the anoxic phase, phosphorus concentration in the wastewater under treatment increased as a result of introducing raw wastewater and secondary phosphorus release to the liquid under anaerobic conditions.As the time of aeration increased, the phosphorus concentration decreased, as they were assimilated by certain bacteria strains (e.g., Acinetobacter sp., in as much as 25% of dry cell weight [3].Aeration of wastewater and activated sludge during 240 minutes resulted in the removal of organic matter (BOD 5 ) with an average rate of 1.5 mg•dm -3 •h -1 (0.39 mg•g DM •h -1 ).During the initial and final 60 minutes of aeration, the removal rate was 2 mg•dm -3 •h -1 (0.53 mg•g DM •h -1 ), while during the other 120 minutes, it dropped to 1 mg•dm -3 •h -1 (0.26 mg•g DM •h -1 ).During this phase removal of organic matter referred to as COD occurred at an average rate of 1.6 mg•dm -3 •h -1 .It should be underlined that during the first 60 minutes, the rate of impurities removal was 4.7 mg•dm -3 •h -1 (1.24 mg•g DM •h -1 ), while during the next 180 minutes of aeration, it decreased in the range from 0.9 to 0.1 mg•dm -3 •h -1 .An increase in concentration TOC was caused by carbon as similation by heterotrophic organisms.Organic matter can also be precipitated and adsorbed in activated sludge [3].In sedimentation phase in SBR in Sokolka separation of solids as settled sludge is allowed to provide clarified supernatant under quiescent condition.No influent or effluent currents interfere with settling process.In some cases gentle mixing during initial stages of settling allows clearer effluent and more concentrated sludge.Phase of sedimentation of the activated sludge in SBR caused the re-release of total phosphorus in wastewater, however, it was much lower (rate 0.1 mg•dm -3 •h -1 ), than during aeration phase.The rate of changes in organic compounds defined by BOD 5 during this phase amounted to 0.18 mg•g DM •h -1 and the increased amounts of organic substances (as COD) in wastewater was recorded (4.1 mg•dm -3 •h -1 ).The concentration of TOC decreased during the settling phase, which may have been caused by carbon consumption by denitrificators [3].
In decantation phase in SBR in Sokolka clarified treated supernatant is allowed to remove within the predetermined cycle time.Decanters are used in this process which may be of floating type or fixed type.During decantation phase total phosphorus removal and

Table 1 .
Types of municipal wastewater and sludge subjected to physicochemical determination.

Table 2
shows the average flows and loads of municipal and dairy wastewater flowing into the sewage treatment plant according to operational data from 2010-2014.

Table 2 .
Quantities and loads of contaminants in dairy and municipal wastewater.

Table 3 .
Production characteristics of dairy processing plant. ) r X -rate of the parameter change in wastewater, mg•g DM -1