Electrochemical Method for Heavy Metals Detection by Inhibition of Acetylcholinesterase Immobilized on Pt-nanoparticles Modified Graphite Electrode
Department of Physical Chemistry, University “Babes-Bolyai”, RO-400028 Cluj-Napoca, ROMANIA
The optimization and the characterization of a new amperometric biosensor based on acetylcholinestrase (AChE), immobilized on a graphite electrode modified with Pt-nanoparticles (PtNP), are reported. The G/PtNP-AChE biosensor was used for heavy metals detection. The degree of inhibition (%I), the kinetic constants of the inhibition process, as well as the influence of the PtNP presence on these parameters were estimated.
Key words: Heavy metals / acetylcholinesterase / inhibition / wastewater
© Owned by the authors, published by EDP Sciences, 2013
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