Speciation analysis of arsenic compounds in the serum and urine of a patient with acute arsine poisoning
1 Laboratory of Environmental Toxicology and Carcinogenesis, Nihon University School of Pharmacy, Funabashi, Chiba 274-8555, JAPAN
2 Department of Hygiene and Preventive Medicine, Showa University, School of Medicine, Tokyo, 142-8555, JAPAN
3 Department of Infectious Disease, Yokohama Municipal Citizen’s Hospital, Yokohama 240-8555, JAPAN
4 Research Center for Occupational Poisoning, Kansai Rosai Hospital, Japan Labour Health and Welfare Organization, Amagasaki 660-8511, JAPAN
5 Department of Preventive Medicine and Environmental Health, Graduate School of Medicine, Osaka City University, Osaka 545-8585, JAPAN
Arsine is one of the most potent hemolytic agents. It is important to clarify arsine metabolism as well as its chemical interactions with biological components. The aim of the present study was to clarify arsine metabolism by arsenic speciation analysis in serum and urine from an acute poisoning patient with hematuria, anemia, and renal and liver dysfunction. Speciation analysis of arsenics in serum and urine was performed using HPLC-ICP-MS. The total arsenic (T-As) concentration in serum was 244.8 μg/l at admission and 97.1 μg/l at discharge. In the speciation analysis, four kinds of As compounds derived from arsine metabolism were detected in serum and urine. The concentration of arsenite (AsIII), arsenate (AsV), monomethylarsonic acid (MMA), and dimethylarsinic acid (DMA) in serum at admission were 45.8, 5.2, 17.9 and 9.3 μg/l, respectively. The concentrations of AsIII, AsV, and MMA decreased with biological half time (BHT) of 30.1, 43.0, and 96.3 h, respectively. Only DMA was increased at discharge. The urinary AsIII, AsV, MMA and DMA concentrations were 223.0, 12.1, 317.5 and 1053.5 μg/l at discharge, and decreased with BHT of 15.1, 20.8, 14.7, and 16.0 d, respectively. The results indicate that arsine was quickly metabolized to AsIII and subsequently up to DMA, with the result that the toxic effects of inorganic arsenic were added to those of arsine toxicity.
Key words: Acute poisoning / arsine / blood / speciation analysis
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