| Issue |
E3S Web Conf.
Volume 125, 2019
The 4th International Conference on Energy, Environment, Epidemiology and Information System (ICENIS 2019)
|
|
|---|---|---|
| Article Number | 04002 | |
| Number of page(s) | 6 | |
| Section | Environmental Health and Toxicology | |
| DOI | https://doi.org/10.1051/e3sconf/201912504002 | |
| Published online | 28 October 2019 | |
Characterisation of Collagen from the Skin of Catfish (Pangasius sp) for Innovative PVA-Collagen Nanofiber
1 Physic Department, Science and Mathematics Faculty, Diponegoro University, Semarang - Indonesia
2 Chemical Engineering Department, Engineering Faculty, Diponegoro University, Semarang – Indonesia
3 Nursing Department, Medical Faculty, Diponegoro University, Semarang - Indonesia
* Corresponding author: erdinalmuqoddas.2017@fisika.fsm.undip.ac.id
Preparation of PVA-Collagen nanofiber plaster production begin by repairing the skin of catfish, then collagen is extracted with distilled water. Extracted collagen need to be frozen dry so that collagen can be obtained in the form of sheets, powder, or crystals. The next step is the production of PVA-Collagen nanofiber, a 10% solution of polyvinyl alcohol (PVA) is mixed with a previously prepared extracted collagen and stirred until it forms a homogeneous mixture. Next, PVA-Collagen nanofiber is formed by the electrospinning method. The results of characterization using FTIR found three main groups in six variations: amide group A; amide I; and amide II. Amide A has a wave number in the range 3300-3440 cm-1, amide I is in the range 1625-1690 cm-1, and amide II is in the range of 1480-1575 cm-1. Characterization was carried out to determine the surface morphology of the observation in the sample using Photomicrograph (200 µm) with magnification 10x, produced fiber diameter formed in variations of PVA-Collagen 1% 116-120 µm while in the variation of PVA-Collagen 1% + Chitosan 10 drops in diameter 41-50 µm.
Key words: Collagen / Nanofiber / Electrospinning
© The Authors, published by EDP Sciences, 2019
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