| Issue |
E3S Web Conf.
Volume 131, 2019
2nd International Conference on Biofilms (ChinaBiofilms 2019)
|
|
|---|---|---|
| Article Number | 01011 | |
| Number of page(s) | 5 | |
| DOI | https://doi.org/10.1051/e3sconf/201913101011 | |
| Published online | 19 November 2019 | |
Biosynthesis of Red Elemental Selenium Protein
1
School of chemistry and chemical engineering, Zhongkai University of Agriculture and Engineering, Guangzhou Guangdong 510225, China
2
School of food and pharmaceutical engineering, Zhaoqing College, Zhaoqing Guangdong 526061, China
3
School of materials science, Northwestern Polytechnical University, Xi’an Shanxi 710072, China
a Corresponding author: Jinhui Huang jinhui11818@outlook.com
The red elemental selenoprotein was biosynthesized by active dry yeast in this study. Through a single factor experiment and L9(34)-orthogonal test fermentation condition optimization, the selenium content was 15.72 mg/g under optimal condition. The optimum culture condition was as follows: the sodium selenite concentration (0.8 mg/mL), culture time (36 h), the yeast dosage (5 g), pH (5.5), culture temperature (30°C), and the medium Baume degree (8 °Bé). The analysis of amino acid species, content and morphology of the product showed that the red elemental selenoprotein was successfully synthesized.
© The Authors, published by EDP Sciences, 2019
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