| Issue |
E3S Web Conf.
Volume 131, 2019
2nd International Conference on Biofilms (ChinaBiofilms 2019)
|
|
|---|---|---|
| Article Number | 01012 | |
| Number of page(s) | 7 | |
| DOI | https://doi.org/10.1051/e3sconf/201913101012 | |
| Published online | 19 November 2019 | |
Resolving Trypanosoma brucei Flagellar Structure by Cryo-Electron Tomography
Fudan University, Biological Sciences Department, 220 Handan Rd., Yangpu District, Shanghai, China
a Corresponding author: jenniferxddai@yeah.net
Trypanosoma brucei is a unicellular eukaryote that can cause human African trypanosomiasis, which has continued to evolve and spread. The key feature of these parasites is that they have a flagellum consists of a typical 9 + 2 axoneme and a lattice-like paraflagellar rod (PFR). It attached to the cell body and is responsible for cell motility, cytokinesis, and morphogenesis. The present study demonstrates the detailed structure and defines the length of the axoneme and three domains of the paraflagellar rod (PFR) using cryo-electron tomography of Trypanosoma brucei flagella. The performed analysis revealed the double-headed structure of the outer-arm dynein, the internal structure of PFR and identified repeating structure in the flagella. Since these structures are critical to the pathogenicity of Trypanosoma brucei, and understanding their organization would help in finding treatments against African trypanosomiasis.
© The Authors, published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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