| Issue |
E3S Web Conf.
Volume 78, 2019
2018 International Seminar on Food Safety and Environmental Engineering (FSEE 2018)
|
|
|---|---|---|
| Article Number | 01002 | |
| Number of page(s) | 5 | |
| Section | Nutrition and Health, Medical Treatment and Biomedical Engineering | |
| DOI | https://doi.org/10.1051/e3sconf/20197801002 | |
| Published online | 15 January 2019 | |
MKL-1 regulates the stem cell marker. CD44 in breast cancer cells
1
Institute of Biology and Medicine, Wuhan University of Science and Technology, P.R.China, 430000
2
Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education and Tianjin, College of Biotechnology, Tianjin University of Science and Technology, P.R.China, 300457
3
Shenzhen Ritzcon Biological Technology Co., LTD, Shenzhen, Guangdong, P.R.China, 518000
* Corresponding author: xinghualiao@hotmail.com
CD44, cluster of differentiation 44 is a typical marker of stem cells. At present, it has been found that CD44 is prevalent in various human malignant tumors, but its expression regulation mechanism is still not clear. The initiation of gene expression, the modification of RNA levels, and the regulation of protein levels are the main factors affecting the expression level of genes, and the most critical one is the regulation of gene expression by signaling pathways. Up to now, there has been no report on the role of MKL-1 in the cloning of the cd44 promoter. Therefore, this study intends to clone the cd44 gene promoter, construct its luciferase reporter gene vector, transfect the MKL-1 overexpression vector, and analyze how it affects transcriptional activity, in order to further study the expression regulation of cd44. The mechanism provides a powerful tool in the future.
© The Authors, published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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