Analysis of microbial community and pollutants removal performance in the simulated reaction pool by immobilized microbe technology

¹ College of Environment of Guangxi University, Nanning 530004, China
² College of Chemistry and Chemical Engineering, Hunan University, Hunan 410082, China

^* Corresponding author: xianping@gxu.edu.cn

Abstract

In this lab-scale simulation, the performance of aerated immobilized microbe (IM) in in-situ pond sedimentation remediation, and the microbial community was studied. It is known for the results that total organic matter (TOM), total nitrogen (TN) and total phosphorus (TP) in the sediment can be effectively reduced by aerated immobilized microbe technology. The polluted raw water, classified as inferior Class-Ⅴ according to the surface water environment quality standard (GB3838-2002), upgraded to Class-III in 30-days remediation with TOM, TN and TP removal rates of 33.35%, 43.35% and 23.20%, respectively. In contract, the control group without the assistant of aerated immobilized microbe still remains at Class-IV. High-throughput sequencing analysis reveals that immobilized microbe technology could improve the species diversity and abundance of the flora, and enhance the similarity between the sediment and the overlying water in the system. The dominant bacteria of the overlying water were Perlucidibaca, Limnohabitans, Bacillus, Hydrogenophaga, Mycobacterium etc. And the dominant bacteria of the sediment were Methanolinea, Methanoregula, Candidatus Competibacter, Methanosaeta etc. Total phosphorous, certified by the redundancy analysis, is the key environmental factor of microbial community structure in both overlying water and sediment.