Immobilization of Isocitrate Dehydrogenase on Mesoporous Silica Foam for Carbon Dioxide Capture

¹ Department of Bioproducts and Biosystems Engineering, University of Minnesota, Saint Paul, MN 55108, USA
² Department of Petroleum Engineering, University of Houston, Houston, TX 77023, USA
³ Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA 70803, USA

Abstract

Carbon capture can be realized effectively through isocitrate dehydrogenase reaction and the reaction rate was strongly affected by the environmental parameters such as pH and temperature. Enzyme immobilization was conducted to improve the enzyme stability during the capture process. By simply adsorbing enzyme on the surface of mesoporous silica foam, enzyme stability against temperature, pH and shear stress was improved. The immobilization process can be completed in 5 mins, and 0.87 U enzyme activity was kept for each gram of immobilization material. After 10 cycles, more than 50 percent of enzyme activity remained. The reusability and improved stability made immobilized ICDH a better candidate for large-scale application of carbon capture.