| Issue |
E3S Web Conf.
Volume 185, 2020
2020 International Conference on Energy, Environment and Bioengineering (ICEEB 2020)
|
|
|---|---|---|
| Article Number | 02004 | |
| Number of page(s) | 4 | |
| Section | Energy Saving and Environmental Protection Technology | |
| DOI | https://doi.org/10.1051/e3sconf/202018502004 | |
| Published online | 01 September 2020 | |
A network-based analysis of key pharmacological pathways of Corilagin acting on Breast cancer and experimental validation
1 College of Life Science and Technology, Dalian University, Liaoning 116622, China
2 Department of Engineering Physics, Institute of Medical physics, Tsinghua University, Beijing 100084, China
* Corresponding author: wanghuiguo163@163.com
The combination of network pharmacological methods and experimental verification has revealed the underlying molecular mechanism of Corilagin in the treatment of breast cancer. In this study, we used Swiss Target Prediction database network to predict the possible candidate targets and pathways of Coilagin, and collect breast cancer targets and pathways of CooLGeN. The result of network pharmacological screen indicated that Bcl is a key pathway for Corilagin in the treatment of breast cancer. The CCK-8 experiment also proved that Corilagin could inhibit the proliferation of MCF-7 cells. Western Blot was used for the validation of network pharmacological results, which showed that Corilagin could increase the apoptosis rates (Bcl-2/Bax) of MCF-7 cells, and up-regulates the apoptotic protein Caspase-3 and changes the Bcl-2 family proteins expression in MCF-7 cells. All results suggest that Corilagin has the potential therapeutic effects on breast cancer, and may be useful for adjuvant chemotherapy.
© The Authors, published by EDP Sciences, 2020
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