| Issue |
E3S Web Conf.
Volume 185, 2020
2020 International Conference on Energy, Environment and Bioengineering (ICEEB 2020)
|
|
|---|---|---|
| Article Number | 04015 | |
| Number of page(s) | 4 | |
| Section | Chemical Engineering and Food Biotechnology | |
| DOI | https://doi.org/10.1051/e3sconf/202018504015 | |
| Published online | 01 September 2020 | |
Fifty-Hertz low frequency magnetic field modifies sarcoplasmic reticulum function
1 Physics Teaching Center of Zhuhai College of Jilin University, 519041, Guangdong Zhuhai China
2 Department of clinical laboratory, Huashan Hospital, Fudan University, 200040, Shanghai China
Among the putative mechanisms, by which extremely low frequency magnetic field (ELF-MF) modify calcium metabolism is that of affecting Ca2+ fluxes across cell membrane or internal Ca2+ stores. To test this hypothesis, whether ELF MF can modulate Ca2+ fluxes of isolated skeletal muscle sarcoplasmic reticulum vesicles (SR) was investigated in the present study. The Ca2+ transport and Ca2+-Mg2+-ATPase activity were observed by means of dynamic Ca2+ dye spectrum, the function of ryanodine receptor (RyR1) was monitored by [3H]-ryanodine binding assay. The membrane fluidity of SR was measured by fluorescence polarization. 50 Hz 0.4 mT MF exposure for 30 min led to a significant decrease in the Ca2+ uptake initial rate and Ca2+-Mg2+-ATPase activity compared to sham exposed SR. These results strongly suggest that prevention of SR Ca2+ uptake by ELF MF exposure was due to the inhibition of Ca2+-Mg2+- ATPase activity, and the increase of SR Ca2+ release was due to the activation of RyR1.
© The Authors, published by EDP Sciences, 2020
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