Issue |
E3S Web Conf.
Volume 213, 2020
2nd International Conference on Applied Chemistry and Industrial Catalysis (ACIC 2020)
|
|
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Article Number | 01033 | |
Number of page(s) | 6 | |
Section | Industrial Catalysis and Chemical Substance R&D and Application | |
DOI | https://doi.org/10.1051/e3sconf/202021301033 | |
Published online | 01 December 2020 |
Determination of Residues of two Aminoglycoside antibiotics and five Quinololine antibiotics and Their Metabolites in Penaeus vannamei by Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry
1
Agriculture Products Processing Research Institute of South China, Zhanjiang 524001, China
2
Agricultural technology extension Center, Zhanjiang 524001, China
3
Laboratory of Quality & Safrty Risk Assessment on Agro-products Processing (Zhanjiang), Ministry of agriculture
* Corresponding author’s e-mail: 76462276@qq.com
An ultra performance liquid chromatography – electrospray ionization triple-quadruple tandem mass spectrometric(UPLC–ESI MS /MS) method was developed for determining Quinololine antibiotics (Carbadox,, Quinocetone, Mequindox, Olaquindox, Cyadox, Tilmicosin, Tylosin, quinoxaline-2-carboxylic acid, 3-methylquinoxaline-2-carboxylic acid) and aminoglycoside antibiotics(Tilmicosin, Tylosin) residues in Penaeus. vannamei, Samples were extracted with acidified acetonitrile, then cleaned up with hexane. The separation of targets was carried out on a Waters BEH C18 column using acetonitrile and 0. 1% fomicacid as mobile phase. Analyte identification and quantification were performed by MS/MS under multiple-reaction monitering(MRM) mode with the external standard method. The results showed that there were good linear relationships between peak area and concentrations of nine targets in the range of 2.5 ~ 50 μg/L, with correlation coefficients more than 0. 999 and The limits of detection(LOD) were in the range of 0. 1 ~ 0.9 μg/kg. the recoveries ranged from 80.2% ~ 94.0%, with RSDs of 1.7%~8.4%. The method is simple, accurate and precise, and It could satisfy the requirement of mass and fast analysis in the laborator.
© The Authors, published by EDP Sciences, 2020
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