| Issue |
E3S Web Conf.
Volume 251, 2021
2021 International Conference on Tourism, Economy and Environmental Sustainability (TEES 2021)
|
|
|---|---|---|
| Article Number | 02030 | |
| Number of page(s) | 5 | |
| Section | Environmental Ecological Analysis and Sustainable Development Research | |
| DOI | https://doi.org/10.1051/e3sconf/202125102030 | |
| Published online | 15 April 2021 | |
Design of influenza diagnostic instrument based on biochemiluminescence
1 Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 518055, China
2 University of Chinese Academy of Sciences, 518055, China
3 Department of Equipment, Nanfang Hospital, Southern Medical University, Guangzhou Guangdong 510515, China
* Corresponding author: 15297818386@163.com
The design principle of a biochemiluminescent instrument is presented. The instrument diagnoses influenza based on detection of the flu viral neuraminidase activity. The instrument uses a photomultiplier tube (PMT) as sensor, with 24 test channels. The reaction disk is driven by step motor to rotate, where the reagent is incubated. Finally, PMT detects the number of photons emitted by the reagent and calculates the enzyme activity. The correlation coefficient (R2) of 2.83 to 5.06 log TCID50/ml influenza virus was 0.9967; the detection limits of the two positive virus samples were TCID50/mL and 953 TCID50/mL, respectively, The positive predictive value (PPV) was 100%, and the negative predictive value (NPV) was 98.90% in clinical evaluation. According to the evaluation, the designed biochemiluminescent instrument can well match with the reagents, and meet with the performance requirements of clinical setting.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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