| Issue |
E3S Web Conf.
Volume 254, 2021
International Scientific and Practical Conference “Fundamental and Applied Research in Biology and Agriculture: Current Issues, Achievements and Innovations” (FARBA 2021)
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|
|---|---|---|
| Article Number | 05004 | |
| Number of page(s) | 7 | |
| Section | Biologization of Agriculture as a Mechanism for the Formation of Soil Fertility | |
| DOI | https://doi.org/10.1051/e3sconf/202125405004 | |
| Published online | 05 May 2021 | |
Antimycotic activity of bacterial strains against the pathogen of grape necrotic leaf spotting Alternaria sp.
1 FSBSI «North Caucasian Regional Research Institute of Horticulture, Viticulture, Wine-making», 40-Years of Victory, 39, 350901, Krasnodar, Russia
2 FSBSI «Federal scientific center «V.S. Pustovoit All-Russian Research Institute of Oil crops», Filatova street, 17, 350038, Krasnodar, Russia
* Corresponding author: rita-miss@mail.ru
Species of the genera Bacillus Cohn and Pseudomonas Migula are successfully used as biocontrol agents for many mycopathogens, including Alternaria sp. To assess the bacteria antagonistic potential against Alternaria sp, we used the method of counter cultures on various nutrient media – universal and specialized. Of the 24 bacterial strains of the genus Bacillus, 18 strains were developed antagonistic activity to Alternaria sp. after 10 days of co-cultivation on the Tylona-3 medium. The maximum sterile zone (8.0 mm) was formed by bacterial strains (R-9, 5B-1, 01 cor f Bacillus sp.). 3 strains of 9 bacteria of the genus Pseudomonas, developed antagonistic activity to Alternaria sp. on Kinga B medium - 14-3 Pseudomonas sp., Oif 2-1 Pseudomonas sp., 14-4 Pseudomonas sp. The maximum antibiotic activity on potato-sucrose agar was established in strains 3-3 Bacillus sp., and K 1-1 Bacillus sp. (sterile zone 7.5 mm). All the tested bacteria most often caused one sign of antagonism – antibiosis.
© The Authors, published by EDP Sciences, 2021
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