| Issue |
E3S Web Conf.
Volume 254, 2021
International Scientific and Practical Conference “Fundamental and Applied Research in Biology and Agriculture: Current Issues, Achievements and Innovations” (FARBA 2021)
|
|
|---|---|---|
| Article Number | 08014 | |
| Number of page(s) | 5 | |
| Section | Scientific Support for the Innovative Development of Animal Husbandry | |
| DOI | https://doi.org/10.1051/e3sconf/202125408014 | |
| Published online | 05 May 2021 | |
Correlation between mitochondrial dysfunction of spermatozoa and their biological adequacy
1 Peoples’ Friendship University of Russia, 117198 Moscow, Russian Federation
2 Federal Research Center for Animal Husbandry named after L.K. Ernst, 142132 Dubrovitsy, Moscow region, Russian Federation
* Corresponding author: rystsova-eo@rudn.ru
One of the most significant indicators affecting male fertility is the sperm nuclear and mitochondrial DNA fragmentation index (DFI). DNA damage depends on biotic and abiotic factors, leading to oxidative stress (O.S.). This research aimed to investigate the relationship between mitochondrial dysfunction of spermatozoa and their biological adequacy. The research material was frozen-thawed sperm samples from the Ayrshire, Russian Black Pied Holstein, Russian Red Pied Holstein, Limousin, and Polled Russian breeding bulls. Assessments of mobility, morphology, and fragmentation index were performed using computer-assisted sperm analysis (CASA). It was found that there is a negative correlation between sperm activity and mitochondrial dysfunction with the correlation coefficient r = -0.24. The incidence of abnormal spermatozoa correlated with sperm dysfunction r = 0.77. The nDNA fragmentation index in chromatin varied from 0 to 25%.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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