| Issue |
E3S Web Conf.
Volume 290, 2021
2021 3rd International Conference on Geoscience and Environmental Chemistry (ICGEC 2021)
|
|
|---|---|---|
| Article Number | 01026 | |
| Number of page(s) | 4 | |
| Section | Environmental Chemistry and Chemical Technology Application | |
| DOI | https://doi.org/10.1051/e3sconf/202129001026 | |
| Published online | 14 July 2021 | |
Purification of tubulin and microtubules from mouse brain
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, China
* Corresponding author: 760101559@qq.com
Purified tubulin or microtubules are widely used for in vitro assays related to their function, such as activities of their modifying enzymes and motor protein activity. This paper gave a review for purification tubulin and microtubules (MTs) using two different methods. One method of purification MTs depended on polymerization and depolymerization. MTs were polymerized at 37 °C with taxol in the presence. Another method is to purify tubulin by affinity column chromatography. Stu2p, a kind of MAPs, is able to bind with C-terminal of tubulin. Recombinant Stu2p was purified from expressing bacteria. Then Stu2p was coupled with NHS-activated resins, which were used to purify tubulin from mouse brain. Tubulins purified in this way can also be polymerized at 37 °C.
© The Authors, published by EDP Sciences, 2021
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