Proteomic and metabolomic analysis of the Eskimo-1 line on the effect of low positive temperatures

¹ Laboratoryof plant’s Proteomics and Metabolomics Center of Genomics and Bioinformatics of the Academyof Sciences of the Republic of Uzbekistan Universitet str., 2A, 111215, Tashkent province, Uzbekistan
² Tashkent State Agrarian University, University str., 2, 100140, Tashkent, Uzbekistan

^* Corresponding author: rakhmatova_nodira@mail.ru

Abstract

Low temperatures are a significant stressor that adversely affects plant growth and productivity. They cause mechanical damage to the aerial parts of the plant, while salinity and drought disrupt the ionic and osmotic balance of the cell. Cold damage can also occur due to extremely low temperatures that lead to irreversible damage to plant organs. Consequently, low temperatures are a limiting environmental factor for agricultural productivity. This study aimed to investigate the response of cotton seedlings (lines ESKIMO1 and Coker-312) at the 2-4 true leaf stage to low temperatures. The content of free proline and endogenous salicylic acid was measured after the plants were exposed to temperatures below the critical threshold (+4 0 С, +10 0 С, +15 0 С, and +24 0 С) for 24 and 48 hours in climatic chambers. Analysis of the results revealed changes in the amino acid and phytohormone content after 24 hours of exposure to low temperatures. Based on these findings, we conclude that these substances participate in the adaptation process, protecting plants from the damaging effects of abiotic temperature factors. These results have implications for improving plant growth and productivity under low temperature conditions.