The effect of immunotherapy on the state of the antioxidant system in radiation damage in rats

¹ Federal center for toxicological, radiation and biological safety, Nauchniy Gorodok Street 2, Kazan city 420075, Russia
² Kazan Innovative University named after V.G. Timiryasov, Moskovskaya Street 42, Kazan city, 420111, Russia

^* Corresponding author: edie.plotnikova@bk.ru

Abstract

Immunotropic drugs (such as sera, blood plasma and immunoglobulins) have polyfunctional activity with anti-infectious, antitoxic, homeostasis-regulating properties. Based on this, we conducted the present study with the purpose to evaluate the therapeutic effect of anti-cadmium -anti-radiation serum (AARS) on the level of radiation-chemical damage. Modeling of experimental radiation-chemical damage was carried out by preliminary 4-time subcutaneous injection of cadmium chloride solution (CdCl₂) at doses of 1/500 LD₅₀, followed by radiation exposure of 90 outbred white rats at doses of 7.0 and 9.0 Gy (LD₅₀ and LD₁₀₀, respectively). It was found that a single subcutaneous injection of TDC at a dose of 25 mg/kg of live weight 24 hours after the combined radiation-toxic damage had a radioprotective and antitoxic effect, increasing the survival rate of animals affected by lethal doses of CdCl₂ and γ-rays. Increase in the survival rate of animals treated with the test agent after exposure to two-factor damage was due to inhibition of the concentration of BFR-active toxic compounds and reciprocal increase in the synthesis of metallothioneins and SOD. Thus, the use of immunotropic agent of anti-cadmium anti-radiation serum (AARS) after exposure to two-factor combined radiation-chemical damage provided 90% survival rate by induction of increased formation of metallothioneins and SOD, inhibition of redox-cycled toxic radicals formation and neutralization of cadmium toxicity with the help of anti-cadmium antibodies.