| Issue |
E3S Web Conf.
Volume 437, 2023
The 5th International Conference on Green Environmental Engineering and Technology (IConGEET2023)
|
|
|---|---|---|
| Article Number | 02011 | |
| Number of page(s) | 9 | |
| Section | Environmental Management and Protection | |
| DOI | https://doi.org/10.1051/e3sconf/202343702011 | |
| Published online | 16 October 2023 | |
Evaluating Recovery Control Concentrations of Bovine Coronavirus (EVAg 015V-02282) Used for SARS-CoV-2 Wastewater Surveillance
1 National Instiute for Research and Development in Environmental Protection, Splaiul Independenţei 294, Bucharest, Romania, 060031
2 Sustainable Environment Research Group (SERG), Centre of Excellence Geopolymer and Green Technology (CEGeoGTech), Universiti Malaysia Perlis, Arau 02600 Perlis, Malaysia
* Corresponding author: raluca.prangate@incdpm.org
SARS-CoV-2 is a pathogenic strain of coronavirus which causes acute respiratory disease in humans. Community spread of COVID-19 was difficult to assess in the beginning of the pandemic, so new methods of detecting the virus had to be discovered. For this reason, national wastewater surveillance systems were implemented in order to detect the spread of SARS-CoV-2 virus. However, to establish such systems, there’s a need to standardize protocols for coronavirus concentration, as well as finding the optimal titre of BCoV, generally used as a recovery control. In our study, we used a strain of Betacoronavirus 1 (EVAg 015V-02282) provided by EVAg as our process control. In order to set up which concentration of the Bovine coronavirus (BCoV) will give the most conclusive results, we diluted the virus three times by the decimal method before running the samples in the QIAcuity Digital PCR (dPCR). The evaluation was made so that the BCoV concentration could be used for future studies.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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