Acute Hepatopancreatic Necrosis Disease (AHPPND) control in Whiteleg shrimp (Litopenaeus vannamei Boone) used inulin source from Dahlia uber (Dahlia variabilis Willd.)

National Research and Innovation Agency (BRIN)

^* Corresponding author: arieftaslihan@yahoo.com

Abstract

The effect of Inulin derived from Dahlia tubers as a functional diet on the growth of white legs shrimp was studied to see its impact on AHPND infection. The research was conducted in a fiber tank filled with 500 L of seawater sterilized with chlorine and stocked with 100 PL30 shrimps/container. Shrimp were treated with feed containing Inulin extract from dahlia tubers at a dose of 2.5%, 5.0%, and 5.0% commercial Inulin and compared with a control. Each treatment was given 2 replications. Shrimp were then challenged with Vibrio parahaemolyticus strain AHPND at a density of 10⁶ CFU/mL. The live shrimp were then processed histopathologically to view the microanatomical gut structure (midgut). The results showed that the mean survival of the shrimp in the control and 2.5% Inulin treatment groups was 97.3 ± 1.15% and 97.3 ± 2.3%, respectively, and the 5% Inulin treatment and commercial Inulin were each 96.9 ± 2.7%. Histopathological observations on Inulin treatment of 2.5% dahlia tuber extract and commercial Inulin showed that shrimp intestine had more lactic acid bacteria abundance than control and 5% Inulin extract. In conclusion, shrimp treated with inulin can reduce the risk of shrimp being infected with AHPND.