A Comparison of ethanol production utilizing separated hydrolysis fermentation and simultaneous saccharification and fermentation methods applied to Tanduk banana peel waste

¹ Politeknik Negeri Bandung, Chemical Engineering Department, 40559 West Java, Indonesia
² Institut Teknologi Bandung, Bioengineering Department, 40116 West Java, Indonesia

^* Corresponding author: keryanti@polban.ac.id

Abstract

In 2021, Indonesia’s banana production is expected to reach 8.74 million tons. This substantial volume of bananas will generate a notable quantity of banana peel waste, constituting approximately one-third of the total banana fruit mass. Remarkably, banana peel waste retains 18.5% carbohydrates, which can be effectively converted into glucose, serving as a valuable feedstock for ethanol production. This research is focused on the ethanol production derived from Tanduk banana peel waste, employing a comparative analysis of the Separated Hydrolysis Fermentation (SHF) and Simultaneous Saccharification and Fermentation (SSF) techniques. Additionally, it explored varying raw material concentrations, specifically 200, 250, and 300 g/L. The SSF method integrated the use of cellulase enzymes, while the SHF method relied on HCl for the hydrolysis process. Microbial fermentation was facilitated by Saccharomyces cerevisiae, with a fermentation duration of 96 hours and sample collection at 12-hour intervals. The results demonstrated that higher concentrations of banana peel waste yield increased glucose production during the hydrolysis process, consequently leading to greater ethanol produced. The concentration of 300 g/L banana peel waste is optimal for producing ethanol in both procedures. However, the SHF approach demonstrates a superior ethanol concentration of 51%, surpassing the 33.7% achieved by the SSF method.