Characterization of Nanoemulsion Non-Ionic and Cytotoxicity Test on T47d Cells Ethanol Fraction of Barleria Prionitis L.

Universitas Muhammadiyah Magelang, Magelang, Indonesia

^* Corresponding author: fitrianayuliastuti@unimma.ac.id

Abstract

The formulation of the ethanol fraction of landep leaves identified bioactive compounds and evaluated anticancer activity on T47D breast cancer cells in vitro and was formulated as a nano emulsion to increase the solubility of drugs that are difficult to dissolve in water by increasing bioavailability. The aim of this research is to determine the anticancer activity and to characterize the Barleria prionitis ethanol fraction formulated in nano emulsion form and to determine the physical properties of the nano emulsion preparation. Landep leaf ethanol fraction nanoparticles were formulated with a surfactant formulation of 0.1% Tween 80 and 5% glycerol as a co surfactant, with a homogenization technique using a magnetic stirrer at a speed of 15,000 Rpm for 120 minutes. The results of the anticancer activity test showed that the IC50 value for T47D cells was 150.2 µg/mL. the nanoparticles formed were characterized using % transmittance value, and Particle Size Analyzer (PSA) and PDI to determine particle size. size. The best nanoparticle test results were obtained in the formula with a dose of 2000ug/mL which had physical quality properties with a particle size of 276.2 nm; The polydispersity index is 0.324 with nanoparticle size and has the most homogeneous particle distribution. The stability of the preparation using organoleptic, pH and viscosity tests gave quite stable results for 14 days so that it could be continued with stability tests to determine shelf life.