Adaptive and viable characteristics of microclonal seedlings of hyperhylophyte of Suaeda arcuate under in vitro conditions

Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Uzbekistan

^* Corresponding author: xalbekova.xulkar@mail.ru

Abstract

This article presents data on the introduction of in vitro culture and microclonal propagation of plants identified in the group of hyperhalophytes belonging to the Suaeda arcuata Bunge. This study was carried out to optimize the composition of nutrient media for the main stages of reproduction in vitro, as well as studies on the rooting and adaptation of regenerants for species of the Suaeda arcuata from axillary or apical buds, but more often from stem segments with a node. In this work, hormones of the cytokinin and auxin series, or a combination of them, were added to the nutrient environment for growth activation. The cultivation of regenerates on the environment 1/2 MS + 1 mg/l 6-BAP + 0.3 mg/l IAA + 2,4-D showed the best effect on the growth of regenerants, created the possibility of obtaining the maximum amount of biomass. Our study indicated that Suaeda arcuata is a highly salt-tolerant species that survived at 300 mM NaCl most conducive to plant growth, where the quantity of Pro and Met were bigger than the control.