Effectiveness of compositions based on thiosulfonates and biosurfactants for plant protection against phytopathogens

¹ Samarkand State University of Veterinary Medicine, Livestock and Biotechnology, Samarkand, Uzbekistan
² Kazan Federal University, 18 Kremlin Street, Kazan, 420008, Russia
³ Belgorod State Agricultural University named after V.Ya. Gorin, 308511, Mayskiy, Belgorod region, Russia
⁴ Kuban State Agrarian University named after I.T. Trubilin, 13 Kalinin Street, Krasnodar, 350044, Russia

^* Corresponding author: urijsyromatnikov86@gmail.com

Abstract

The study investigates the effectiveness of new compositions based on thiosulfonates and biogenic surfactants in combating phytopathogenic microorganisms such as Agrobacterium tumefaciens and Clavibacter michiganensis. The use of a rhamnolipid biocomplex in combination with thiosulfonates significantly enhanced antimicrobial activity and reduced the minimum inhibitory and bactericidal concentrations of the agents. Combinations of rhamnolipids with thiosulfonates proved more effective than thiosulfonates alone, demonstrating a reduction in the required concentrations by approximately 50%. The effect of the compositions on the permeability of microbial cell membranes was studied by measuring the amount of extracellular protein released. The results showed that rhamnolipid biocomplex increased membrane permeability, which in turn heightened bacterial sensitivity to the applied biocidal agents. The proposed environmentally friendly compositions can be used to create new plant protection products, contributing to a reduction in the chemical load on the environment and minimizing the risk of developing biocide- resistant forms of phytopathogens.