Potential biodegradable bacteria and their identification

¹ National Academy of Sciences of the Kyrgyz Republic, Kyrgyzstan, 720071, Bishkek
² Kyrgyz-Turkish University "Manas", Kyrgyzstan, 720038, Bishkek
³ Kyrgyz National Agrarian University named after K.I.Skryabin, Kyrgyzstan, 720005, Bishkek

^* Corresponding author: ch.omurgazieva@gmail.com

Abstract

The article describes soil bacterial isolates isolated from technogenic zones of Kyrgyzstan, possessing high oil-oxidizing and bioemulsifying activity, capable of decomposing petroleum hydrocarbons in the temperature range of 4 - 39°C at pH 4 - 9 in the presence of 18% salt. Our research has confirmed the possibility of bioremediation of the damage caused to soils as a result of chemical pollution of soils (lands) due to spillage of oil products on the territory of the oil depot in Balykchy city of Kyrgyzstan. The used bacterial consortium consisting of spore-forming bacteria of Bacillus genus: B1-025-4(2), B1-05-3(2), B1-025-5 had high oil-degrading activity both at low (4°C) and moderate (18-25°C) temperatures. In this work, special attention was paid to the molecular genetic properties of 4 bacterial strains isolated from oil-contaminated and different types of soils. The maximum biodegradability of these strains for many hydrocarbons (hexadecane, benzene, naphthalene, diesel fuel, gasoline) was also noted. Based on physiological-biochemical and molecular genetic analysis, the isolated petroleum destructor bacteria were found to belong to Bacillus pumilus (strains B1-05-3(2) and B1-025-4(2), B1-025-5) and Bacillus amyloliquefaciens (strain H-5-2). These selected strong bacterial strains are of great interest for biotechnological applications as effective microorganisms for environmental cleaning. Keywords: bacteria-destructors, effective strains, oil-contaminated soil, bioremediation, identification, molecular methods.