Study on the submerged fermentation process producing perylenequinones derivatives by shiraia sp. AL18

¹ Shandong University of Technology, School of Life Science, 266 Xin cun xi road Zibo, 255049, China
² Shandong University of Technology, Institute for Anti-aging & Regenerative Medicine, 266 Xin cun xi road Zibo, 255049, China

^* Corresponding author: lumingfeng4659@sina.com

Abstract

The perylenequinones derivatives (PQDs) are potential photosensitizers, which have a high effective light-induced biological activity, such as anti-HIV, antimicrobial and anticancer activities. In this work, the submerged fermentation process of filamentous fungus shiraia sp. AL18 producing PQDs in a stirring fermentation tank was investigated through single factor optimization. The effect of the stirring speed (450 r/min, 500 r/ min and 550 r/min at ventilation quantity for volumes of air per volume of liquid per minute (VVM) = 1:1) and ventilation quantity (VVM = 1:0.8, 1:0.65 and 1:0.5 at stirring speed of 500 r/min) on the process of submerged fermentation by stirring fermentation tank in batch fermentation was investigated using the monosyllabic test. The results suggest that the optimum stirring speed for biomass is 500 r/min and the optimum ventilation quantity for biomass is VVM = 1:0.65, and the maximum dry cell weight (DCW) reached to 19.67 g/L. The stirring speed for the optimum yield of PQDs is 500 rpm and the ventilation quantity for the optimum yield of PQDs is VVM=1: 0.8, and the maximum yield of PQDs reached to 0.3218 g/L. The yield of PQDs was correlated with pH value, dissolved oxygen (DO) value, the content of reduced sugar in the period of fermentation.