| Issue |
E3S Web Conf.
Volume 78, 2019
2018 International Seminar on Food Safety and Environmental Engineering (FSEE 2018)
|
|
|---|---|---|
| Article Number | 02003 | |
| Number of page(s) | 4 | |
| Section | Food Safety, Extraction and Food Engineering | |
| DOI | https://doi.org/10.1051/e3sconf/20197802003 | |
| Published online | 15 January 2019 | |
Optimization of Cephalosporin C Acylase Immobilization
1
Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China
2
Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, Beijing 100083, China
3
Department of Biological Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China
* Corresponding author: yhchang@ustb.edu.cn (Y. Chang); luohui@ustb.edu.cn (H. Luo)
Cephalosporin C Acylase (CCA), the key biocatalyst in one-step enzymatic production of 7- amino cephalosporanic acid (7-ACA), was immobilized by amino-activated carrier (LX-1000HA) and epoxy-activated carriers (ES-103B, LX-1000EPC), and the activity was assayed. ES-103B carriers showed an advantage than the others. The amount of free enzyme and salt concentration were tested, and the optimum conditions were 1400U/g and 0.9M. In addition, two different methods (by shaker and packed column) were used in CCA immobilization, and the results demonstrated that the former obtained a higher immobilized CCA activity.
© The Authors, published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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