E3S Web Conf.
Volume 78, 20192018 International Seminar on Food Safety and Environmental Engineering (FSEE 2018)
|Number of page(s)||4|
|Section||Food Safety, Extraction and Food Engineering|
|Published online||15 January 2019|
Optimization of Cephalosporin C Acylase Immobilization
Department of Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China
2 Beijing Key Laboratory of Resource-oriented Treatment of Industrial Pollutants, Beijing 100083, China
3 Department of Biological Science and Engineering, University of Science and Technology Beijing, Beijing 100083, China
Cephalosporin C Acylase (CCA), the key biocatalyst in one-step enzymatic production of 7- amino cephalosporanic acid (7-ACA), was immobilized by amino-activated carrier (LX-1000HA) and epoxy-activated carriers (ES-103B, LX-1000EPC), and the activity was assayed. ES-103B carriers showed an advantage than the others. The amount of free enzyme and salt concentration were tested, and the optimum conditions were 1400U/g and 0.9M. In addition, two different methods (by shaker and packed column) were used in CCA immobilization, and the results demonstrated that the former obtained a higher immobilized CCA activity.
© The Authors, published by EDP Sciences, 2019
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