| Issue |
E3S Web Conf.
Volume 78, 2019
2018 International Seminar on Food Safety and Environmental Engineering (FSEE 2018)
|
|
|---|---|---|
| Article Number | 02005 | |
| Number of page(s) | 4 | |
| Section | Food Safety, Extraction and Food Engineering | |
| DOI | https://doi.org/10.1051/e3sconf/20197802005 | |
| Published online | 15 January 2019 | |
Rapid Determination of Chloramphenicol in Tilapia by ultra-high performance liquid chromatography-mass spectrometry
1
Agriculture Products Processing Research Institute, Chinese Academy of tropical Agriculture Sciences, Zhanjiang 524001, Guangdong, China
2
Laboratory of Quality and Safety Risk Assessment on Agro-products Processing(Zhanjiang), Ministry of Agriculture and Rural Affairs of PRC, Zhanjiang 524001, Guangdong, China
* Corresponding author: zjyangcl@126.com
A rapid method for the determination of residues of chloramphenicol in tilapia by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. The samples were extracted with acetonitrile, and separated on a C18 column using methanol-water solution as mobile phase, and then detected under ESI- multiple reaction monitoring mode. The method showed a good linearity for the analysts over the range of 0.1-100μg/L. The detection limits were 0.10μg/kg. The recoveries ranged from 88.6% to 108% at spiked concentrations with the relative standard deviations lower than 5%. The results shows that this method has the advantages of easy to operate, fast to perform, with high sensitivity and accuracy, and it is suitable for detection of residues of chloramphenicol in tilapia.
© The Authors, published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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