| Issue |
E3S Web Conf.
Volume 98, 2019
16th International Symposium on Water-Rock Interaction (WRI-16) and 13th International Symposium on Applied Isotope Geochemistry (1st IAGC International Conference)
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|---|---|---|
| Article Number | 01051 | |
| Number of page(s) | 5 | |
| Section | Geological Evolution of Water-Rock System: Mechanisms, Processes, Factors, Stages. The Session Dedicated to Stepan Shvartsev’s Memory | |
| DOI | https://doi.org/10.1051/e3sconf/20199801051 | |
| Published online | 07 June 2019 | |
Influence of changes in microbial cell membrane composition on isotopic fractionation of nitrate during denitrification
1
Chair of Hydrogeology, Department of Civil, Geo and Environmental Engineering, Technische Universität München (TUM), Arcisstrasse 21, 80333 München, Germany
2
Chair of Analytical Chemistry and Water Chemistry, Institute of Hydrochemistry, Technische Universität München (TUM), Marchioninistr. 17, 81377 München, Germany
3
Department Environmental Biotechnology, Helmholtz Centre for Environmental Research-UFZ, Permoserstrasse 15, 04318 Leipzig, Germany
* Corresponding author: anja.wunderlich@tum.de
Pure cultures of Thauera aromatica were stressed by the addition of the toxic solvents 1-octanol and 4-chlorophenol causing adaptive modifications in their cell-membrane fatty acid composition. At the same time, we investigated the isotopic fractionation in δ15N-NO3- during denitrification. We observed a change in the degree of saturation (DoS) of the bacteria as a reaction to solvent stress and a higher degree of saturation was directly correlated to the concentration of solvents in the cell membrane. The enrichment factor ε15N-NO3- during denitrification showed a linear dependency to the DoS as well as to the membrane solvent concentration. Denitrifying bacteria thus may express less negative ε15N-NO3- when they are exposed to environmental stress that changes their cell membrane composition.
© The Authors, published by EDP Sciences, 2019
This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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