E3S Web Conf.
Volume 98, 201916th International Symposium on Water-Rock Interaction (WRI-16) and 13th International Symposium on Applied Isotope Geochemistry (1st IAGC International Conference)
|Number of page(s)||5|
|Section||Geological Evolution of Water-Rock System: Mechanisms, Processes, Factors, Stages. The Session Dedicated to Stepan Shvartsev’s Memory|
|Published online||07 June 2019|
Influence of changes in microbial cell membrane composition on isotopic fractionation of nitrate during denitrification
Chair of Hydrogeology, Department of Civil, Geo and Environmental Engineering, Technische Universität München (TUM), Arcisstrasse 21, 80333 München, Germany
2 Chair of Analytical Chemistry and Water Chemistry, Institute of Hydrochemistry, Technische Universität München (TUM), Marchioninistr. 17, 81377 München, Germany
3 Department Environmental Biotechnology, Helmholtz Centre for Environmental Research-UFZ, Permoserstrasse 15, 04318 Leipzig, Germany
* Corresponding author: firstname.lastname@example.org
Pure cultures of Thauera aromatica were stressed by the addition of the toxic solvents 1-octanol and 4-chlorophenol causing adaptive modifications in their cell-membrane fatty acid composition. At the same time, we investigated the isotopic fractionation in δ15N-NO3- during denitrification. We observed a change in the degree of saturation (DoS) of the bacteria as a reaction to solvent stress and a higher degree of saturation was directly correlated to the concentration of solvents in the cell membrane. The enrichment factor ε15N-NO3- during denitrification showed a linear dependency to the DoS as well as to the membrane solvent concentration. Denitrifying bacteria thus may express less negative ε15N-NO3- when they are exposed to environmental stress that changes their cell membrane composition.
© The Authors, published by EDP Sciences, 2019
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