| Issue |
E3S Web Conf.
Volume 164, 2020
Topical Problems of Green Architecture, Civil and Environmental Engineering 2019 (TPACEE 2019)
|
|
|---|---|---|
| Article Number | 06029 | |
| Number of page(s) | 11 | |
| Section | Agriculture and Biotechnologies | |
| DOI | https://doi.org/10.1051/e3sconf/202016406029 | |
| Published online | 05 May 2020 | |
Biosynthesis lipase from the fungus Penicillium hordei isolated from waste fat
1 Saint-Petersburg State Institute of Technology, 190013, 26 Moskovsky prospect, St. Petersburg, Russia
2 Kashkin Research Institute of Medical Mycology, North-Western State Medical University named after I.I. Mechnikov, 194291, 1/28 Santiago de Cuba street, St. Petersburg, Russia
* Corresponding author: malexpush@bk.ru
The article provides a method of isolating a lipase producer and identification methods by classical methods in microbiology of an isolated micromycete producing lipase. The species affiliation of Penicillium hordei has been determined. The temperature of micromycete cultivation was studied, as well as the dynamics of Penicillium hordei lipase biosynthesis. Screening plans were also carried out (Plackett-Berman Plan), then optimization according to the steep climb plan and an experiment based on the plan of a full-factor experiment (three-level CFE) was also conducted to determine the optimal concentrations that are significant for lipase biosynthesis of components. A mathematical model is constructed that describes the relationship between the influence of two significant factors on the volumetric activity of P. Hordei culture fluid. The most optimal composition for the culture fluid for lipase biosynthesis, Penicillium hordei micromycete, was compiled.
© The Authors, published by EDP Sciences 2020
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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