Microflora microbiological characteristics of saline soils

Kuban State Agrarian University named after I. T. Trubilin, 13, Kalinina str., 350044, Krasnodar, Russia

^* Corresponding author: prihodkoigor2012@yandex.ru

Abstract

The purpose of current work was isolation of halophilic bacteria from saline lands in some regions of the Krasnodar Territory, after mycological analysis, there followed the study and characterization of microorganisms with further creation on effective biocomplex basis, contributing to restoration of arid and saline irrigated areas. Soil samples were taken from saline lands of the Krasnodar Territory, the salt composition of which was studied in the work process. There were determined total titer of microorganisms and the titer of nitrogen-fixing bacteria on the Corresponding selective media in the samples. Twenty-five pure bacterial cultures were isolated from the samples and its morphophysiological and biochemical properties, as well as its nitrogenfixing activity, were studied. As a result, seven cultures with relatively high nitrogen-fixing activity were selected, as well as resistance to various environmental factors, particularly, to high concentrations of NaCl, рН alkaline values and to sharp temperature drops. After appropriate phylogenetic analysis, selected crops may become a potential target for creating a complex biological fertilizer, contributing to the restoration of dry and saline lands and increase their fertility.