| Issue |
E3S Web Conf.
Volume 254, 2021
International Scientific and Practical Conference “Fundamental and Applied Research in Biology and Agriculture: Current Issues, Achievements and Innovations” (FARBA 2021)
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|
|---|---|---|
| Article Number | 04005 | |
| Number of page(s) | 8 | |
| Section | The Role of Biotechnology in Obtaining Pure Virus-free Material | |
| DOI | https://doi.org/10.1051/e3sconf/202125404005 | |
| Published online | 05 May 2021 | |
Some aspects of clonal micropropagation of Amelanchier Medik. genus representatives
1 FSBIS Main Botanical Garden named after N.V. Tsitsin, RAS, Laboratory of Plant Biotechnology, 127276, Moscow, Russia
2 FSBIS Main Botanical Garden named after N.V. Tsitsin, RAS, Laboratory of Cultured Plants, 127276, Moscow, Russia
* Corresponding author: katyaraeva@rambler.ru
The article presents studies of organogenesis peculiarities of varieties belonging to various species of Amelanchier Medik. genus in in vitro culture at the stages of micropropagation, rooting and depositing. Mineral composition and growth regulator for active adventive microshoots formation were determined. The optimal auxin source for rhizogenesis of various Amelanchier alnifolia varieties has been established. Conditions for explants' preservation in aseptic conditions for 12 months have been revealed. Different effects of cytokinin type substances on shadberry regeneration potential under aseptic conditions were noted. Analysis of regenerants' morphometric indicators showed the effectiveness of using 6-benzylaminopurin (BAP) in the concentration of 1.0 mg/L for shadberry varieties' clonal micropropagation and indolebutyric acid (IBA) at 1.0 mg/L concentration for microshoots' rooting.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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