| Issue |
E3S Web Conf.
Volume 254, 2021
International Scientific and Practical Conference “Fundamental and Applied Research in Biology and Agriculture: Current Issues, Achievements and Innovations” (FARBA 2021)
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|
|---|---|---|
| Article Number | 06006 | |
| Number of page(s) | 6 | |
| Section | Botanical Gardens, Arboretums - Centers for the Study and Conservation of Biodiversity | |
| DOI | https://doi.org/10.1051/e3sconf/202125406006 | |
| Published online | 05 May 2021 | |
Collection of rare and endangered plant species in the meristem bank of the RAS Main Botanical Garden
1 RAS Main Botanical Garden named after N. V. Tsitsin, Laboratory of Plant Biotechnology, 127276, Moscow, Russia .molkanova@mail.ru
2 RAS Main Botanical Garden named after N. V. Tsitsin, Laboratory of Cultural Plants, 127276, Moscow, Russia.
* Corresponding author: molkanova@mail.ru
Results of many research years on in vitro formation and conservation of genetic plants' bank of rare and endangered species in the Main Botanical Garden of the Russian Academy of Sciences. Peculiarities of plants' cultivation and conservation in vitro related to different families were revealed. The main methodological aspects at the stages of obtaining axenic culture, micropropagation and long-term deposition are reflected. The main criterion in choosing the optimal explant type for both taxa introduction into the culture in vitro and for long-term reproduction and conservation is the plants’ life form. The nutrient media formulations and cultivation factors for slow explants’ growth of the studied crops and conservation of their viability were optimized. It was revealed that when selecting optimal conditions of cultivation and conservation it is necessary to consider the biological features of the taxon.
© The Authors, published by EDP Sciences, 2021
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