| Issue |
E3S Web Conf.
Volume 265, 2021
Actual Problems of Ecology and Environmental Management (APEEM 2021)
|
|
|---|---|---|
| Article Number | 04010 | |
| Number of page(s) | 5 | |
| Section | Environmental Management | |
| DOI | https://doi.org/10.1051/e3sconf/202126504010 | |
| Published online | 03 June 2021 | |
UPGMA - analysis of type II CRISPR RNA-guided endonuclease Cas9 homologues from the compost metagenome
1 G.K. Scriabin Institute of Biochemistry and Physiology of Microorganisms RAS, Pushchino, Russia
2 Pushchino State Institute of Natural Science, Pushchino, Russia
3 College of Life Sciences, Shanghai Normal University, Shanghai, China
* Corresponding author: dr.zimin8@yandex.ru
Metagenomic approaches provide access to the genetic diversity of the environment for biotechnological applications, allowing the discovery of new enzymes and new pathways for numerous catalytic processes. Five new putative type II CRISPR-Cas9 DNA endonucleases were identified from the compost community using the DELTA-BLAST algorithm. It was determined using phylogenetic UPGMA analysis that four of these potential enzymes are similar to those of the Bacteroidetes. Protein structural modeling confirmed the data of DELTA-BLAST and UPGMA analysis. These new five proteins found may be promising for genome editing in termoresistant Actinomyces.
© The Authors, published by EDP Sciences, 2021
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