E3S Web Conf.
Volume 265, 2021Actual Problems of Ecology and Environmental Management (APEEM 2021)
|Number of page(s)||7|
|Published online||03 June 2021|
The compost metagenome as a source of T4 bacteriophage pyrimidine dimer glycosylase homologues
1 G.K. Scriabin Institute of Biochemistry and Physiology of Microorganisms RAS, Pushchino, Russia
2 Pushchino State Institute of Natural Science, Pushchino, Russia
3 College of Life Sciences, Shanghai Normal University, Shanghai, China
* Corresponding author: Firetiger2011@yandex.ru
Compost is a promising source of thermotolerant enzymes for their application in biotechnology. Homologues of bacteriophage T4 DNA glycosylase can find their application in pharmaceuticals and perfumery. Five homologues of glycosylase of pyrimidine dimers of bacteriophage T4, a product of the denV gene, were found by comparing using the DELTA-BLAST algorithm with the compost metagenome proteins. Phylogenetic analysis of the found sequences of enzyme homologues was carried out using the Maximum Likelihood algorithm in the MegaX software package. Thus, an interesting spectrum of promising proteins, homologues of the repair enzyme, DNA glycosylase of pyrimidine dimers of bacteriophage T4, was found. After structural modeling, they can be tested for their thermal stability and tested as a basis for therapeutic and prophylactic drugs.
© The Authors, published by EDP Sciences, 2021
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