Effects of sophoroflavonoside and narcissin flavonoids on mitochondrial dysfunction and antioxidant enzyme activity in rat liver poisoned with galoxyfop-r-methyl pesticide

¹ Andijan State University, Andijan, 170100, Uzbekistan
² Tashkent State Pedagogical University, Tashkent, 100070, Uzbekistan
³ National University of Uzbekistan, Tashkent, 100174, Uzbekistan
⁴ Tashkent State Agrarian University, Tashkent, 100140, Uzbekistan

^* Corresponding author: mashxura.parpieva1001@mail.ru

Abstract

This study delves into the accumulation of residues in the liver tissue of rats exposed to the galoxyfop-R-methyl pesticide. The investigation also evaluates the presence of malondialdehyde (MDA), a lipid peroxidation (LPO) product, in the liver mitochondrial membrane, along with the activity of antioxidant enzymes catalase, superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxidase (GP). The study further explores the impact of sophoraflavonoloside (SFL) and narcissin flavonoids on enzyme activity over a dynamic span of 10 to 40 days. In the experimental design, rats in the study group were subjected to galoxyfop-R-methyl pesticide at a dosage equivalent to LD50/10 through a specialized probe. Subsequently, the concentration of the pesticide residues in liver tissue was measured on the 5th, 10th, 20th, 30th, and 40th days post-pesticide exposure. The research also probes into the content of malondialdehyde (MDA) in the liver mitochondrial membrane, as well as the activity of antioxidant enzymes, within the context of SFL. This meticulous examination aids in comprehending the dynamics of enzyme responses and oxidative stress modulation in the liver of rats subjected to galoxyfop-R-methyl pesticide. By investigating the intricate interactions between pesticide exposure, enzyme activity, and antioxidant mechanisms, this study contributes to the broader understanding of the potential effects and counteractive measures concerning pesticide-related challenges in biological systems.