Content of cytochrome P-450 in microsomal fractions of the liver of pregnant rats and their embryos

¹ Andijan State University, Andijan, 170100, Uzbekistan
² Tashkent State Agrarian University, Tashkent, 100140, Uzbekistan
³ Tashkent State Pedagogical University, Tashkent, 100070, Uzbekistan

^* Corresponding author: tds.bio@mail.ru

Abstract

This article presents a comprehensive examination of the impact of the pesticide “dropp” on the lipid peroxidation (LPO) of microsome membranes and the levels of cytochrome P-450 enzyme within microsomal fractions of pregnant rats and their developing embryos. The study delves into the intricate biochemical responses within these biological systems following exposure to the pesticide. The research uncovers a significant intensification of NADP.Nand ascorbate-dependent lipid peroxidation in the liver microsomes of both pregnant rats and their embryos upon exposure to the “dropp” pesticide. This heightened lipid peroxidation is particularly notable on the 3rd day of pregnancy, accompanied by an elevated concentration of malondialdehyde (MDA), a marker of oxidative stress. Moreover, the study reveals a noteworthy impact of “dropp” on the content of cytochrome P-450 enzyme within the microsomal fraction of the liver in pregnant rats. This enzyme, which plays a vital role in various metabolic processes, experiences a reduction in concentration upon pesticide exposure. The effect is most pronounced on the 3rd and 19th days of pregnancy, highlighting specific time windows of vulnerability. Collectively, these findings provide a nuanced understanding of the biochemical repercussions of “dropp” pesticide exposure on the microsomal membranes and enzyme dynamics within pregnant rats and their developing embryos.