Levan Production in Shake Flask and Fermenter Influence of Feeding Strategy on Levan Yield and Molecular Weight Distribution

¹ Biomass conversion and Bioproducts Laboratory, Center for Bioenergy, School of Chemical & Biotechnology, SASTRA Deemed University, Thirumalaisamudram, Tamil Nadu, India
² Department of Biosystems Engineering, Auburn University, Auburn, AL, USA 36849
³ Biorefinery and Process Automation Engineering Center, Department of Chemical and Process Engineering, The Sirindhorn ThaiGerman International Graduate School of Engineering, King Mongkut’s University of Technology North Bangkok, Thailand

^* Corresponding author: vponnu@chem.sastra.edu

Abstract

Effect of feeding strategy on levan production was studied in a shake flask and a 5 L lab scale fermenter. In a shake flask, levan specific substrate yield (YP/S) increased from 0.35 g levan/g sucrose to 0.48 g levan/g sucrose with repeated batch feeding. In a 3 L reactor, levan productivity of 7.18 g/L h was obtained with the fed-batch mode of fermentation. The gel permeation chromatography results indicate that higher initial sucrose concentration under the fed-batch mode operation resulted in the formation of low molecular weight fractions (4 and 10 kDa). Thus, fed-batch fermentation favors levan production resulting in higher yield and productivity and also affects the molecular weight distribution of the biopolymer.