| Issue |
E3S Web Conf.
Volume 442, 2023
International Seminar on Fish and Fisheries Sciences (ISFFS 2023)
|
|
|---|---|---|
| Article Number | 02009 | |
| Number of page(s) | 7 | |
| Section | Aquaculture | |
| DOI | https://doi.org/10.1051/e3sconf/202344202009 | |
| Published online | 09 November 2023 | |
The effect of alginate concentration on the viability of probiotics (Bacillus subtilis) in synbiotic microcapsule beads after incubation at 70°C
1 Doctoral Study Program in Fisheries Science, Faculty of Marine Science and Fisheries, Hasanuddin University, 90241 Makassar, South Sulawesi, Indonesia
2 Department of Fisheries Science, Faculty of Marine Science and Fisheries, Hasanuddin University, 90241 Makassar, South Sulawesi, Indonesia
3 Research Center for Fishery, National Research and Innovation Agency, 16911 Republic of Indonesia
4 Faculty of Fisheries and Marine Science, Dayanu Ikhsanuddin University, 93711 Baubau
* Corresponding author: bungatampangallo@yahoo.com
High temperatures in feed molding machines can cause a decline in probiotic populations in fish feed. Adding alginate in probiotic preparations is expected to wrap probiotics so that when added to the feed before pelleting, it does not experience a drastic decrease. This study investigated the decline in probiotic populations in fish feed caused by high temperatures in feed molding machines and evaluated the use of alginate in probiotic preparations to prevent drastic decreases in viability. The study aimed to determine the viability of Bacillus sp. in synbiotic microcapsule beads with varying levels of alginate concentration after exposure to 70°C. The research used four treatments and three replicates with a randomized complete design. The results showed that the alginate concentration affected the diameter of the beads of synbiotic microcapsules, but it did not significantly affect the viability of Bacillus sp. The viability of probiotics in alginate beads after exposure to 70°C was 5.182±0.527 - 5.720±0.072 log cfu/ml. Therefore, using alginate in probiotic preparations can be considered as a reference for adding probiotics to fish feed before pelleting to maintain the viability of the probiotics in high-temperature conditions.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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