| Issue |
E3S Web Conf.
Volume 442, 2023
International Seminar on Fish and Fisheries Sciences (ISFFS 2023)
|
|
|---|---|---|
| Article Number | 02010 | |
| Number of page(s) | 6 | |
| Section | Aquaculture | |
| DOI | https://doi.org/10.1051/e3sconf/202344202010 | |
| Published online | 09 November 2023 | |
Development of freeze-dried bivalent fish vaccine product with skimmed milk as stabiliser
1 Research Center for Veterinary Science, Research Organization for Health, National Research and Innovation Agency, Indonesia
2 Research Center for Fisheries, Research Organization for Earth and Maritime, National Research and Innovation Agency, Indonesia
3 Department of Fisheries, Faculty of Agriculture, Gadjah Mada University, Indonesia
* Corresponding author: desysugiani@gmail.com
Bacterial vaccines have been tested for their effectiveness in protecting fish from potential virulent diseases. The current vaccine preparations are mostly in liquid form, which affects prices due to an increase in the cost of shipment, the stability of liquid products also has limitations in long-term storage. This study aims to develop a method of preparation of bivalent vaccine through freeze dry method with skimmed milk as a filler for optimization of the vaccine products. The preparation of bivalent vaccine by freeze dry method can reduce 96.67% of total weight compared to vaccines in liquid form. The quality test showed that bivalent vaccine products by freeze dry method with skimmed milk filler containing bacteria Aeromonas hydrophila and Streptococcus agalactiae are passed the sterility and viability test. The application of the vaccine can provide a positive response to increase the antibody titer and other hematological parameter responses and increase the survival rate of fish. The development of freeze-dried vaccine products can optimize fish vaccines as a recommended product in fish disease management.
© The Authors, published by EDP Sciences, 2023
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