| Issue |
E3S Web of Conf.
Volume 444, 2023
4th International Conference on Agribusiness and Rural Development (IConARD 2023)
|
|
|---|---|---|
| Article Number | 04035 | |
| Number of page(s) | 12 | |
| Section | Agricultural Technology and Smart Farming | |
| DOI | https://doi.org/10.1051/e3sconf/202344404035 | |
| Published online | 14 November 2023 | |
A paper stick test kit for rapid detection of total microbial contaminants in fresh milk
Research Center for Agroindustry, National Research and Innovation Agency (NRIA) Soekarno Integrated Science Center, Jl. Raya Jakarta-Bogor km 46, Cibinong 16911, Bogor, Indonesia
* Corresponding author: misg001@brin.go.id
The way to determine the total microbial contaminants in fresh milk is by the Total Plate Count (TPC) method. The TPC method requires 48 hours, high analytical skills, and high analytical costs. The aim of this research was to design a rapid detection tool for microbial contaminants in fresh milk called Paper Stick Test Kit (PSTK), which is fast, cheap, and easy. The stages of research designing PSTK are: (a) making PSTK, (b) testing the optimal time for using PSTK, and (c) making standard color maps. The results of this research are: (a) the best PSTK is made from cardboard with color indicators made from a mixture of bromothymol blue 0.3 g/250 ml solvent and NaOH 0.01 N; drying PSTK is best done in an oven with a temperature of 100oC, for 20 minutes; (b) the optimal time of application of PSTK in fresh milk is 20 seconds; and c) standard color maps are based on the correlation between microbial contaminant levels in fresh milk and color levels in PSTK dipped in microbial contaminated fresh milk.
© The Authors, published by EDP Sciences, 2023
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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