Proline Content, Physiological and Agronomic Characters of Rice (Oryza sativa cv. Inpari Unsoed 79 Agritan) under Treated with PGPR in Saline Medium

¹ Departement of Agrotechnology, Agriculture Faculty, Jenderal Soedirman University, Purwokerto, Central Java, Indonesia 53122
² Faculty of Science and Technology, Peradaban University, Bumiayu, Central Java, Indonesia

^* Corresponding author: purwanto0401@unsoed.ac.id

Abstract

Tidal areas near the coastline are prone to salinity impacts as a negative impact of seawater intrusion which causes dissolved salt levels to increase. The biological approach using PGPR is an environmentally friendly approach. The aim of the study was to examine the effectiveness of useful bacteria from saline soils to increase the resistance of rice plants to salinity stress. The research was carried out from April to October 2022 in the Lab. Agronomy & Horticulture Faperta UNSOED. This research was experimental research using liquid culture techniques for cultivating plants using AB Mix media. The design used was a randomized block design with three replications. The treatments were included Control (P0), Acinetobacter junii (P1), Bacillus tropicus (P2), Acinetobacter schindleri (P3), Pseudomonas stutzeri (P4), Bacillus altitudinis (P5), Bacillus cereus (P6), dan Bacillus subtilis (P7). The results of the study showed that Diazotrophic Bacteria inoculation can increase the resistance of rice plants to rice plants by increasing proline production, increasing net assimilation rates and relative growth rates. Acinetobacter schindleri, Bacillus subtilis and Bacillus tropicus are potential N2 fixing bacterial strains to increase the growth and yield of rice under saline conditions. Treatment of Acinetobacter schindleri was able to provide the highest grain yields reaching 16.95 g/plant.