Different pathways for copper sulphate and copper nitrate antioxidation and organic acid excretion in Typha latifolia?

¹ Helmholtz Zentrum München, German Research Center for Environmental Health, Research unit Microbe-Plant Interactions, Ingolstädter Landstr. 1, 85764 Neuherberg, GERMANY
² Forschungszentrum Jülich GmbH, Institute of Bio- and Geosciences (IBG-2: Plant Sciences), D-52425 Jülich, GERMANY
³ Forschungszentrum Jülich GmbH, Institute of Bio- and Geosciences (IBG-3: Agrosphere), D-52425 Jülich, GERMANY
⁴ Kwame Nkrumah University of Science and Technology, Faculty of Biosciences, Department of Theoretical and Applied Biology Kumasi, GHANA

^a lyudmila.lyubenova@helmholtz-muenchen.de
^b a.kuhn@fz-juelich.de
^c a.hoeltkemeier@fz-juelich.de
^d bips097@yahoo.co.uk
^e ejdbelford@yahoo.co.uk
^f peter.schroeder@helmholtz-muenchen.de

Abstract

The major topic of the present experiment was the investigation of the antioxidative enzymes and the root exudate excretion after plant exposure to copper. The copper was added for each treatment as copper sulphate and copper nitrate in the concentrations of 10 μM, 50 μM and 100 μM, respectively. The plant species chosen for the study was Typha latifolia. The experiment gives insight into the plant responses to different copper supplies during the same conditions of exposure. Remarkable results were obtained during the time course about the excretion of organic acids from Typha latifolia roots during one week of copper exposure. Oxalic acid, malic acid, acetic acid and lactic acid were detected. Interestingly, not all organic acids are excreted from the roots in the first hours after copper addition.