E3S Web of Conferences
Volume 1, 2013Proceedings of the 16th International Conference on Heavy Metals in the Environment
|Number of page(s)||4|
|Section||Biomonitoring / Exposure I|
|Published online||23 April 2013|
Relation between metallothionein, RNA/DNA and heavy metals in juveniles of colossoma macropomun (Cuvier, 1818) in natural conditions
1 Departamento de Biología, Escuela de Ciencias, Universidad de Oriente, Núcleo de Sucre, Venezuela
2 Laboratorio de Ecofisiología y Ecotoxicología, Instituto Oceanográfico de Venezuela
3 Departamento de Bioanálisis, Escuela de Ciencias, Universidad de Oriente, Núcleo de Sucre, Venezuela
4 Departamento de Biología Marina, Instituto Oceanográfico de Venezuela
5 Laboratorio de Inmunotoxicidad, Postgrado en Biología Aplicada, Universidad de Oriente. Núcleo Sucre. Cumaná, Venezuela
Metal content, Metallotionein and physiological condition (RNA/DNA ratio) were analyzed in Cachama, Colossoma macropomum from the Orinoco River. Juvenile samples were dissected and one gram of white muscle gills, kidney and liver was taken. Cu, Cd, Pb, Ni and Fe were analyzed by atomic absorption spectrophotometer using TORT and LUTSI as reference material. Metallothioneins were determined with Ellman´s reagent and RNA/DNA ratio was analyzed by fluorometric method. Results showed that Cd, Cu, Ni, Fe, and Pb levels were significantly higher in liver than muscle. There was no difference statistically between Mts concentrations in liver, kidney and gills; muscle showed the lowest Mts concentrations. Multivariate analysis ACP determined relationship between Mts-L and Cu and Mts-G and Cd. The Mts in muscle was related with Pb and Cd. There was no association between physiological condition and metals concentrations, however, the RNA/ DNA showed relationship with Mts-M and Mts-L
Key words: Metallotionein / RNA/DNA ratio / heavy metals
© Owned by the authors, published by EDP Sciences, 2013
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