The application of impedance measurement to assess biofilm development on technical materials used for water supply system construction

¹ Wroclaw University of Science and Technology, Faculty of Environmental Engineering, Wybrzeze Wyspiańskiego 27, 50-370 Wroclaw, Poland
² Faculty of Microsystem Electronics and Photonics, Wrocław University of Technology, Wybrzeze Wyspianskiego 27, 50-370 Wrocław, Poland

^* Corresponding author: mirela.wolf@pwr.edu.pl

Abstract

The lack of biological stability of water which is introduced into the network, leads primarily to its secondary contamination during transport to the consumer. The water that is biologically unstable creates ideal conditions for colonization of the inner surface of pipelines by microorganisms and adhesion of their products (biocorrosion). The studies was conducted using the identified microorganisms isolated from the water supply network which accounted inocula in continuous culture of biofilm in CDC reactor. As a result of studies it was revealed the presence of biofilm formed on different materials polyethylene, polypropylene, polyvinyl chloride, polybutylene. Microbiological biodiversity of organisms inhabiting a biofilm of the diversity of nucleic acids was used. It was observed the amount of the psychrophilic bacteria oscillation in the effluent from the reactor. It was also determined the affinity of various bacteria to the plastic through adhesion measurement using impedance spectroscopy. For impedance measurements apparatus SIGNAL RECOVERY 7280 DSP LOCK-IN AMPLIFIER was used, recording impedance components (real and imaginary). The results will allow for the creation of biosensor systems that can be used in predicting health risks in connection with drinking water and taking corrective actions.