| Issue |
E3S Web Conf.
Volume 44, 2018
10th Conference on Interdisciplinary Problems in Environmental Protection and Engineering EKO-DOK 2018
|
|
|---|---|---|
| Article Number | 00153 | |
| Number of page(s) | 8 | |
| DOI | https://doi.org/10.1051/e3sconf/20184400153 | |
| Published online | 03 July 2018 | |
Preliminary Microplate Ames MPF™ test use in assessment of mutagenic properties of spider webs
Wrocław University of Science and Technology, Department of Environmental Protection, Wybrzeże Wyspiańskiego 27, 50-370 Wrocław, Poland
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Corresponding author : radoslaw.rutkowski@pwr.edu.pl
Air pollution is one of the most urgent problems of modern world, eventually causing the premature death of millions people every year. One of the burdens due to exposure to air pollutants is a lung cancer. It is necessary to develop new effective methods of carcinogen monitoring. The aim of the study was to evaluate if spider webs are suitable for the assessment of air mutagenicity by Ames MPF™ test. It is the first time spider webs had been incorporated into Ames MPF™ procedure. Webs of two spider species Araneus diadematus and Agelena labyrinthica have been collected at four sites exposed to high pollutants emission. Salmonella typhimurium TA98 strain without metabolic fraction have been used for the assay. All samples exhibited mutagenic activity most likely due to the road traffic. Webs of A. labyrinthica have shown higher mutagenicity effects at the tested sites in comparison to A. diadematus, plausibly because of the longer exposition time. The results are most promising and indicate high potential of combining spider webs and MPF™ procedure for assessing the mutagenic properties of urban air pollution.
© The Authors, published by EDP Sciences, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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