E3S Web Conf.
Volume 44, 201810th Conference on Interdisciplinary Problems in Environmental Protection and Engineering EKO-DOK 2018
|Number of page(s)||8|
|Published online||03 July 2018|
Application of NGS for studying the antibiotic resistant microbiome in Wrocław tap water
Wrocław University of Science and Technology, Faculty of Environmental Engineering, Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland
* Corresponding author: email@example.com
The NGS method was used to investigate the biodiversity of ARB dwelling in tap water, able to form biofilm on bullion media supplemented with antibiotics: β-lactam, fluoroquinolone, 3rd generation cephalosporin and tetracycline. Biofilms formed by cold-and hot-tap water microorganisms were also examined. The presence of 10 ARGs in plasmid DNA of cultivated biomass was tested using a standard PCR. The analyses revealed that the most predominant families were Sphingomonadaceae, Paenibacillaceae, Enterobacteriaceae, Bacillaceae and Sphingomonadaceae in FQ, C3G, T, C-W and H-W samples, respectively (explanations of abbreviations in the text). The ARGs did not correlate with resistance phenotypes.
© The Authors, published by EDP Sciences, 2018
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