| Issue |
E3S Web Conf.
Volume 44, 2018
10th Conference on Interdisciplinary Problems in Environmental Protection and Engineering EKO-DOK 2018
|
|
|---|---|---|
| Article Number | 00161 | |
| Number of page(s) | 8 | |
| DOI | https://doi.org/10.1051/e3sconf/20184400161 | |
| Published online | 03 July 2018 | |
Application of NGS for studying the antibiotic resistant microbiome in Wrocław tap water
Wrocław University of Science and Technology, Faculty of Environmental Engineering, Wyb. Wyspiańskiego 27, 50-370 Wrocław, Poland
* Corresponding author: agata.siedlecka@pwr.edu.pl
The NGS method was used to investigate the biodiversity of ARB dwelling in tap water, able to form biofilm on bullion media supplemented with antibiotics: β-lactam, fluoroquinolone, 3rd generation cephalosporin and tetracycline. Biofilms formed by cold-and hot-tap water microorganisms were also examined. The presence of 10 ARGs in plasmid DNA of cultivated biomass was tested using a standard PCR. The analyses revealed that the most predominant families were Sphingomonadaceae, Paenibacillaceae, Enterobacteriaceae, Bacillaceae and Sphingomonadaceae in FQ, C3G, T, C-W and H-W samples, respectively (explanations of abbreviations in the text). The ARGs did not correlate with resistance phenotypes.
© The Authors, published by EDP Sciences, 2018
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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